A semi-quantitative method for determining the levels of carbonic anhydrase isozymes in individual red blood cells.
نویسندگان
چکیده
A procedure is presented which can be used to analyse the isozymes of carbonic anhydrase (CA I and CA II) as well as other specific soluble proteins of individual erythrocytes. The concentration of red cell CA I in the pig-tailed macaque (Macaca nemestrina) was estimated by the technique employed, as well as the relative concentration in individual cells. In addition, the basic technique was modified to include electrophoresis of the carbonic anhydrases of the individual cells.
منابع مشابه
Testosterone-induced, sulfonamide-resistant carbonic anhydrase isozyme of rat liver is indistinguishable from skeletal muscle carbonic anhydrase III.
Three isozymes of carbonic anhydrase, CA I, CA II and CA III, have been characterized from the tissues of a variety of vertebrate species. CA I (low-activity, sulfonamide-sensitive form) has been found mainly in red cells but also occurs in other tissues (e.g., rumen epithelium, caecum, colonic mucosa, pituitary gland, ciliary body), CA II (high-activity, sulfonamide-sensitive form) is found in...
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Carbonic anhydrase (CA) is a multi-functional enzyme that catalyzes the hydration/dehydration of carbon dioxide. In the red blood cell (rbc), CA is necessary to facilitate the transport of carbon dioxide out of the body. Results from earlier biochemical studies indicate that ancient vertebrates, such as agnathans, possess a low activity rbc CA isozyme, whereas more recently evolved vertebrates,...
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عنوان ژورنال:
- Experimental cell research
دوره 67 2 شماره
صفحات -
تاریخ انتشار 1971